Reading BUB’s DNA sequence: the raw data

Hi again,

Today we’re back with a new episode of the videolog, where I’ll explain what BUB’s DNA sequence looked like fresh off the sequencing machine. A couple of things to note: as our videos will get more and more technical please feel free to leave comments or to contact us if there’s anything you’d like us to clarify. Also, since we can’t include all the technical details, we’ll start leaving links to tools and papers together with the video, so you can follow up on stuff if you’re interested. Hope you enjoy!

More info about:
– the fastq format on wikipedia
– a technical note by Illumina about understanding quality scores
– a table for converting the characters in fastq files into an actual quality score

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